5AYQ
Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of VGSC beta subunits
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-10-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 103.726, 59.282, 42.369 |
Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
Resolution | 29.838 - 1.700 |
R-factor | 0.2047 |
Rwork | 0.203 |
R-free | 0.23050 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.855 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MrBUMP |
Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 100.000 |
High resolution limit [Å] | 1.700 |
Number of reflections | 27557 |
<I/σ(I)> | 33.4 |
Completeness [%] | 99.9 |
Redundancy | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30%(w/v) polyethylene glycol 1500 |