5AYP
Crystal structure of Bacillus stearothermophilus Farnesyl pyrophosphate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-15 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.015, 95.156, 103.349 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.310 |
| R-factor | 0.21324 |
| Rwork | 0.211 |
| R-free | 0.25221 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rtr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.432 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.340 |
| High resolution limit [Å] | 2.310 | 2.300 |
| Number of reflections | 21879 | |
| <I/σ(I)> | 19.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | PEG 400, 0.1M Na accetate, 0.1M LiCl |
