5AYK

Crystal structure of ERdj5 form I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-22
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.10
Spacegroup name	P 1 21 1
Unit cell lengths	91.990, 52.970, 92.900
Unit cell angles	90.00, 113.54, 90.00

Refinement procedure

Resolution	19.951 - 2.250
R-factor	0.216
Rwork	0.214
R-free	0.24320
Structure solution method	MIR
Starting model (for MR)	3apo
RMSD bond length	0.005
RMSD bond angle	0.837
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.140	2.300
High resolution limit [Å]	2.250	2.250
Number of reflections	39380
<I/σ(I)>	11	1.3
Completeness [%]	99.4	94.16
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG8000, cystin