5AYH
Structure of the entire dynein stalk region
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-01 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.90000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 103.325, 103.325, 69.709 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 23.380 - 3.011 |
R-factor | 0.259 |
Rwork | 0.255 |
R-free | 0.29510 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wuq |
RMSD bond length | 0.002 |
RMSD bond angle | 0.592 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.076 | 0.704 |
Number of reflections | 14221 | |
<I/σ(I)> | 36.3 | 3.6 |
Completeness [%] | 96.3 | 96.7 |
Redundancy | 3.2 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | PEG 3350, ammonium phosphate monobasic |