5AY9

Crystal structure of Ruminococcus albus 4-O-beta-D-mannosyl-D-glucose phosphorylase (RaMP1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-08
Detector	RAYONIX MX225HE
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	88.900, 88.900, 286.857
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.003 - 2.500
R-factor	0.2409
Rwork	0.239
R-free	0.28060
RMSD bond length	0.005
RMSD bond angle	1.052
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	46.010
High resolution limit [Å]	2.500
Number of reflections	15552
<I/σ(I)>	14.7
Completeness [%]	99.3
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M sodium nitrate, 0.1M Bis-Tris propane buffer, 20%(w/v) PEG3350