5AY4
Crystal structure of RNA duplex containing C-C base pairs obtained in the presence of Hg(II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.000, 44.540, 27.550 |
Unit cell angles | 90.00, 109.10, 90.00 |
Refinement procedure
Resolution | 26.000 - 1.700 |
Rwork | 0.160 |
R-free | 0.19560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ay3 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.839 |
Data scaling software | XSCALE |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 26.000 | 1.750 | |
High resolution limit [Å] | 1.700 | 7.610 | 1.700 |
Rmerge | 0.088 | 0.052 | 0.323 |
Rmeas | 0.096 | 0.056 | 0.353 |
Total number of observations | 45309 | ||
Number of reflections | 6831 | 81 | 490 |
<I/σ(I)> | 13.38 | 26.04 | 5.58 |
Completeness [%] | 99.5 | 98.8 | 96.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MPD, Spermine, Sodium Nitrate, MOPS |