5AXE
Crystal Structure Analysis of DNA Duplexes containing sulfoamide-bridged nucleic acid (SuNA-NH)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-14 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 23.685, 42.905, 45.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.350 - 0.950 |
| R-factor | 0.126 |
| Rwork | 0.125 |
| R-free | 0.14400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i5w |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data scaling software | Aimless (0.2.17) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.680 | 0.970 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.059 | 0.241 |
| Number of reflections | 29154 | |
| <I/σ(I)> | 27.6 | 7 |
| Completeness [%] | 98.8 | 97.1 |
| Redundancy | 13.7 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MPD |
