5AWS
Crystal structure of the SGIP1 mu homology domain in the P1 space group
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-14 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 |
Unit cell lengths | 37.552, 53.634, 75.199 |
Unit cell angles | 101.87, 86.91, 95.61 |
Refinement procedure
Resolution | 39.054 - 2.001 |
R-factor | 0.2007 |
Rwork | 0.199 |
R-free | 0.24120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5awr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.385 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 34307 |
<I/σ(I)> | 15 |
Completeness [%] | 89.1 |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 293 | PEG 3350, zinc acetate, sodium acetate, sodium iodide |