5AWQ
Arthrobacter globiformis T6 isomalto-dextranse complexed with panose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-16 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | I 41 |
Unit cell lengths | 123.878, 123.878, 85.261 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.840 - 1.480 |
R-factor | 0.16266 |
Rwork | 0.161 |
R-free | 0.18654 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.333 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.480 | 1.480 |
Rmerge | 0.071 | 0.359 |
Number of reflections | 96929 | |
<I/σ(I)> | 47.9 | 6.9 |
Completeness [%] | 91.2 | 100 |
Redundancy | 7.1 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 293 | PEG 8000, sodium acetate buffer, potassium dihydrogenphosphate |