5AWP
Arthrobacter globiformis T6 isomalto-dextranase complexed with isomaltose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
Synchrotron site | Photon Factory |
Beamline | AR-NE3A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-06-16 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | I 41 |
Unit cell lengths | 123.708, 123.708, 84.841 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.450 - 2.000 |
R-factor | 0.15901 |
Rwork | 0.157 |
R-free | 0.19593 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5awo |
RMSD bond length | 0.009 |
RMSD bond angle | 1.353 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.175 | 0.345 |
Number of reflections | 43201 | |
<I/σ(I)> | 40 | 13.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.6 | 293 | PEG 8000, sodium acetate buffer, potassium dihydrogenphosphate |