5AWF
Crystal structure of SufB-SufC-SufD complex from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 119.475, 139.556, 124.684 |
| Unit cell angles | 90.00, 113.10, 90.00 |
Refinement procedure
| Resolution | 41.111 - 2.957 |
| R-factor | 0.1878 |
| Rwork | 0.187 |
| R-free | 0.22620 |
| Structure solution method | MIR |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.241 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.060 | 0.371 |
| Number of reflections | 77331 | |
| <I/σ(I)> | 15.8 | 2.8 |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | pentaerythritol propoxylate (5/4 PO/OH), sodium citrate, potassium chloride |
