5AWD
Crystal structure of human TLR8 in complex with N1-4-aminomethylbenzyl (IMDQ)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-11-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.660, 106.670, 72.160 |
| Unit cell angles | 90.00, 106.24, 90.00 |
Refinement procedure
| Resolution | 69.280 - 2.050 |
| R-factor | 0.21733 |
| Rwork | 0.215 |
| R-free | 0.26901 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.608 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 82.526 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 61824 |
| <I/σ(I)> | 9.6 |
| Completeness [%] | 99.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG3350, potassium formate, sodium citrate |
