5AW9
Kinetics by X-ray crystallography: native E2.MgF42-.2K+ crystal for Rb+ bound crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-23 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 219.972, 50.666, 163.066 |
| Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.218 |
| Rwork | 0.217 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zxe |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.440 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.2) |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.890 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 40891 | |
| <I/σ(I)> | 21 | |
| Completeness [%] | 93.6 | |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7 | 298 | PEG 3000, MPD, potassium acetate, potassium chloride, magnesium chloride, potassium fluoride, MES/TRIS |
