5AVP
Crystal structure of Geodermatophilus obscurus L-ribose isomerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2014-03-20 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 90.533, 123.226, 109.446 |
Unit cell angles | 90.00, 97.38, 90.00 |
Refinement procedure
Resolution | 27.060 - 2.900 |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.29400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ww3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.500 |
Data reduction software | CrystalClear |
Data scaling software | CrystalClear |
Phasing software | MOLREP |
Refinement software | CNS (1.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.060 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.266 | 0.446 |
Number of reflections | 26574 | |
<I/σ(I)> | 5 | 1.31 |
Completeness [%] | 91.8 | 73 |
Redundancy | 3.2 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 1.26M (NH4)2SO4, Tris, Li2SO4 |