5AVJ

Crystal structure of proteinase K from Engyodontium album

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-05-24
Detector	RIGAKU RAXIS VII
Wavelength(s)	1.54
Spacegroup name	P 43 21 2
Unit cell lengths	67.549, 67.549, 107.083
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.542 - 1.450
R-factor	0.164
Rwork	0.163
R-free	0.18380
RMSD bond length	0.008
RMSD bond angle	1.061
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.450
Number of reflections	44672
<I/σ(I)>	12.1
Completeness [%]	99.9
Redundancy	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		291	NaNO3, CaCl2, MES