5AVF
The ligand binding domain of Mlp37 with taurine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-12-13 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.230, 119.050, 138.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.316 - 1.950 |
R-factor | 0.1955 |
Rwork | 0.193 |
R-free | 0.23640 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3c8c |
RMSD bond length | 0.008 |
RMSD bond angle | 1.034 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.300 | 2.060 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.096 | 0.371 |
Number of reflections | 41301 | |
<I/σ(I)> | 13.7 | 5.2 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH 6.5, 15% (w/v) PEG-8000 and 30% MPD |