5AVE
The ligand binding domain of Mlp37 with serine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2013-06-19 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 98.720, 134.220, 50.530 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.721 - 1.800 |
R-factor | 0.1889 |
Rwork | 0.188 |
R-free | 0.21360 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.008 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.721 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.097 | 0.411 |
Number of reflections | 31377 | |
<I/σ(I)> | 11.4 | 4.7 |
Completeness [%] | 99.5 | 99.2 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1M Phosphate-citrate pH 4.2, 10% (w/v) PEG-8000 and 0.2M NaCl |