5AV7
Crystal structure of Calsepa lectin in complex with bisected glycan
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2014-12-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 47.608, 52.836, 54.774 |
Unit cell angles | 90.01, 90.03, 94.05 |
Refinement procedure
Resolution | 54.770 - 1.850 |
R-factor | 0.24712 |
Rwork | 0.245 |
R-free | 0.27877 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ouw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.079 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.880 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.452 | |
Number of reflections | 44270 | |
<I/σ(I)> | 16.7 | 1.9 |
Completeness [%] | 97.0 | 96 |
Redundancy | 1.9 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Hepes-Na (pH 7.5), 3.0 M NaCl |