5AUR
Hydrogenobacter thermophilus cytochrome c552 dimer formed by domain swapping at N-terminal region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-12 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.850, 42.898, 53.591 |
| Unit cell angles | 86.73, 86.51, 89.83 |
Refinement procedure
| Resolution | 50.000 - 1.260 |
| R-factor | 0.18275 |
| Rwork | 0.181 |
| R-free | 0.22909 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ynr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.290 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Rmerge | 0.047 | 0.479 |
| Number of reflections | 74408 | |
| <I/σ(I)> | 17.3 | 2.2 |
| Completeness [%] | 94.7 | 93.5 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 200 mM potassium iodide, 15% (w/v) PEG 3350 |
