5AUK
Crystal structure of the Ga-substituted Ferredoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-01 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 31.942, 31.942, 320.078 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.560 - 1.620 |
| R-factor | 0.19874 |
| Rwork | 0.198 |
| R-free | 0.21923 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1off |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.921 |
| Data reduction software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.650 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.064 | 0.333 |
| Number of reflections | 13883 | |
| <I/σ(I)> | 64.1 | 10.2 |
| Completeness [%] | 96.6 | 91.1 |
| Redundancy | 14.2 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 3.4 M ammonium sulfate, 2% (w/v) benzamidine hydrochloride |
