5AUJ

Pyrococcus furiosus proliferating cell nuclear antigen (PCNA) SeMet derivative

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-13
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	P 63
Unit cell lengths	88.545, 88.545, 62.726
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.170 - 2.500
R-factor	0.2203
Rwork	0.218
R-free	0.27340
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.476
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.500
Number of reflections	19578
<I/σ(I)>	18.1
Completeness [%]	100.0
Redundancy	20

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	ammonium sulfate, 1,4-dioxane, sodium citrate, pH 5.5