5AUI
Crystal structure of Ferredoxin from Thermosynechococcus elongatus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-29 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.1000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 56.441, 53.314, 32.278 |
Unit cell angles | 90.00, 92.38, 90.00 |
Refinement procedure
Resolution | 32.250 - 1.499 |
Rwork | 0.159 |
R-free | 0.18300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b2g |
RMSD bond length | 0.013 |
RMSD bond angle | 1.984 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.499 | 1.499 |
Rmerge | 0.061 | 0.291 |
Number of reflections | 15300 | 2193 |
<I/σ(I)> | 35.5 | 3.9 |
Completeness [%] | 98.8 | 93.6 |
Redundancy | 3.2 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2.6 M Ammonium sulfate, 0.2% benzamidine hydrochloride, 3% (w/v) 1,6-hexanediol |