5AQ5
Structure of the Carboxy-Terminal Domain of the Bacteriophage T5 L- Shaped Tail Fibre
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 86.623, 95.029, 127.748 |
| Unit cell angles | 68.24, 70.20, 83.63 |
Refinement procedure
| Resolution | 23.000 - 2.300 |
| R-factor | 0.22354 |
| Rwork | 0.223 |
| R-free | 0.25228 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4uw7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.326 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.210 |
| Number of reflections | 144381 | |
| <I/σ(I)> | 7.6 | 3 |
| Completeness [%] | 91.3 | 90.5 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-HCL PH 8.5, 8% (W/V) PEG 4000, 10 MM IRON(III) CHLORIDE, 20% (V/V) GLYCEROL |
