5APZ
Thermosinus carboxydivorans Nor1 Tcar0761 residues 68-101 and 191-211 fused to GCN4 adaptors
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-22 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 63 |
| Unit cell lengths | 37.920, 37.920, 179.150 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.830 - 1.600 |
| R-factor | 0.17872 |
| Rwork | 0.177 |
| R-free | 0.21312 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.918 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.300 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.030 | 0.600 |
| Number of reflections | 19111 | |
| <I/σ(I)> | 20.3 | 2.23 |
| Completeness [%] | 99.2 | 96.9 |
| Redundancy | 3.69 | 3.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRI-SODIUM CITRATE PH 4.0, 30 %(V/V) MPD |
