5APY
Sequence MATKDDIAN inserted between GCN4 adaptors - Structure T9(9)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-20 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.770, 35.090, 112.190 |
| Unit cell angles | 90.00, 100.39, 90.00 |
Refinement procedure
| Resolution | 19.520 - 2.000 |
| R-factor | 0.21347 |
| Rwork | 0.211 |
| R-free | 0.25467 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.789 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.500 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.040 | 0.630 |
| Number of reflections | 15902 | |
| <I/σ(I)> | 19.5 | 2.25 |
| Completeness [%] | 98.7 | 97.5 |
| Redundancy | 3.73 | 3.73 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M CITRIC ACID PH 5.0, 20 %(V/V) ISOPROPANOL |
