5APV
Sequence IANKEDKAD inserted between GCN4 adaptors - Structure A9b grey
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-04-02 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 71.090, 34.990, 106.190 |
Unit cell angles | 90.00, 101.71, 90.00 |
Refinement procedure
Resolution | 38.110 - 2.000 |
R-factor | 0.20884 |
Rwork | 0.206 |
R-free | 0.25271 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wpq |
RMSD bond length | 0.006 |
RMSD bond angle | 0.963 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.100 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.520 |
Number of reflections | 32657 | |
<I/σ(I)> | 13.9 | 2.43 |
Completeness [%] | 92.4 | 98.9 |
Redundancy | 3.29 | 3.31 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.2 M SODIUM CITRATE, 0.1 M BIS TRIS PROPANE PH 6.5, 20%(W/V) PEG 3350 |