5APT
Sequence IENKADKAD inserted between GCN4 adaptors - Structure A9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.180, 34.590, 67.510 |
| Unit cell angles | 90.00, 117.66, 90.00 |
Refinement procedure
| Resolution | 32.580 - 1.800 |
| R-factor | 0.20812 |
| Rwork | 0.206 |
| R-free | 0.25635 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wpq |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.877 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.700 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.050 | 0.620 |
| Number of reflections | 24951 | |
| <I/σ(I)> | 14.3 | 2.07 |
| Completeness [%] | 98.9 | 97.4 |
| Redundancy | 3.7 | 3.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.6 M TRI-SODIUM CITRATE PH 6.5 |
