5APS
Sequence IENKKAD inserted between GCN4 adaptors - Structure A7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-27 |
Detector | MARRESEARCH |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 38.240, 38.240, 87.100 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.510 - 1.370 |
R-factor | 0.19749 |
Rwork | 0.195 |
R-free | 0.23823 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wpq |
RMSD bond length | 0.018 |
RMSD bond angle | 1.942 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 18.200 | 1.450 |
High resolution limit [Å] | 1.370 | 1.370 |
Rmerge | 0.050 | 0.760 |
Number of reflections | 16040 | |
<I/σ(I)> | 18.2 | 2.52 |
Completeness [%] | 99.0 | 98.6 |
Redundancy | 6.35 | 6.33 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M CITRIC ACID PH 3.5, 3 M NACL |