5APC
Hen Egg White Lysozyme illuminated with 0.4THz radiation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 297 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.350, 79.350, 37.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.170 - 1.700 |
| R-factor | 0.17368 |
| Rwork | 0.172 |
| R-free | 0.19698 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.731 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.090 | 0.740 |
| Number of reflections | 11830 | |
| <I/σ(I)> | 75.6 | 10.5 |
| Completeness [%] | 91.9 | 66.7 |
| Redundancy | 205 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 50MM NA ACETATE, PH 4.5 1M NACL 25% ETHYLENE GLYCOL 30-100MG/ML HEWL IN 50MM NA ACETATE, PH 4.5 |
