5AP8

Structure of the SAM-dependent rRNA:acp-transferase Tsr3 from S. solfataricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-17
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	49.590, 62.486, 196.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.085 - 2.246
R-factor	0.2245
Rwork	0.224
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NONE
RMSD bond length	0.004
RMSD bond angle	0.769
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.090	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.040	1.410
Number of reflections	29932
<I/σ(I)>	21.39	1.41
Completeness [%]	99.6	98
Redundancy	3.5	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		12.5 % MPD, 12.5 % PEG1000, 12.5 % PEG 3350, 100 MM TRIS/BICINE PH 8.5, 30 MM DIETHYLENE GLYCOL,30 MM TRIETHYLENE GLYCOL, 30 MM TETRAETHYLENE GLYCOL, 30 MM PENTAETHYLENE GLYCOL