5AP1
Naturally Occurring Mutations in the MPS1 Gene Predispose Cells to Kinase Inhibitor Drug Resistance.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-10 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 70.680, 108.380, 115.530 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.340 - 2.050 |
| R-factor | 0.1954 |
| Rwork | 0.195 |
| R-free | 0.21070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c4j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.340 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.040 | 1.260 |
| Number of reflections | 28097 | |
| <I/σ(I)> | 13 | 1 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M MES PH 6.5, 0.2M MGCL2, 10% (W/V) PEG4000, CO-CRYSTALLISED WITH 1MM INHIBITOR |
