5AOZ
High resolution SeMet structure of the third cohesin from Ruminococcus flavefaciens scaffoldin protein, ScaB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 60.427, 60.427, 86.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.540 - 1.140 |
| R-factor | 0.1196 |
| Rwork | 0.118 |
| R-free | 0.14243 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.750 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (AUTOSOL) |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.540 | 1.810 |
| High resolution limit [Å] | 1.140 | 1.140 |
| Rmerge | 0.070 | 1.330 |
| Number of reflections | 58923 | |
| <I/σ(I)> | 18.2 | 1.73 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 10.3 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 45 MG ML PROTEIN IN 0.1 M (NH4)2SO4, 20% PEG 4K, 15% GLYCEROL, pH 7.0 |
