5AOY
Structure of mouse Endonuclease V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.630, 114.030, 54.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.474 - 1.750 |
| R-factor | 0.1661 |
| Rwork | 0.165 |
| R-free | 0.19030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b20 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.194 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.470 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.100 | 0.540 |
| Number of reflections | 32035 | |
| <I/σ(I)> | 11 | 3 |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 4 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
