5AOO
X-ray structure of a human Kobuvirus: Aichi virus A (AiV)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 350.816, 350.816, 350.816 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 71.460 - 2.100 |
| R-factor | 0.3301 |
| Rwork | 0.330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bev |
| RMSD bond length | 0.930 |
| RMSD bond angle | 1.571 |
| Data reduction software | XDS |
| Phasing software | GLRF |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.460 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.170 | 0.870 |
| Number of reflections | 186646 | |
| <I/σ(I)> | 5.5 | 1.1 |
| Completeness [%] | 90.0 | 83.6 |
| Redundancy | 3.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.05M CADMIUM SULFATE 0.1M HEPES PH 7.5 1.0M SODIUM ACETATE |
