5AON
Crystal structure of the conserved N-terminal domain of Pex14 from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Collection date | 2015-06-14 |
| Detector | DECTRIS |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 53.569, 53.569, 342.827 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.571 - 1.646 |
| R-factor | 0.1971 |
| Rwork | 0.195 |
| R-free | 0.23160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ff5 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.899 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.570 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.060 | 0.480 |
| Number of reflections | 37178 | |
| <I/σ(I)> | 24.3 | 6.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 17.4 | 18.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 21% PEG3350, 0.5M AMMONIUM SULFATE, 0.1M TRIS-HCL PH8.5 |
