5ANR
Structure of a human 4E-T - DDX6 - CNOT1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 93.129, 93.129, 175.329 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.324 - 2.102 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.23190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ct4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.035 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.320 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.090 | 1.040 |
| Number of reflections | 51910 | |
| <I/σ(I)> | 17.61 | 1.6 |
| Completeness [%] | 99.7 | 97.4 |
| Redundancy | 9.4 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 50 MM TRIS PH 8.0, 35% MPD |
