5ANH
CRYSTAL STRUCTURE OF LACCASE FROM BASIDIOMYCETE PM1 (CECT 2971)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.440, 175.660, 103.700 |
| Unit cell angles | 90.00, 104.16, 90.00 |
Refinement procedure
| Resolution | 49.180 - 2.492 |
| R-factor | 0.1966 |
| Rwork | 0.193 |
| R-free | 0.27290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENRY 2HRG |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.177 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.120 | 0.480 |
| Number of reflections | 59209 | |
| <I/σ(I)> | 8.82 | 2.07 |
| Completeness [%] | 96.6 | 89 |
| Redundancy | 2.97 | 2.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M TRIS-HCL PH 8.5, 1.6 M AMMONIUM SULFATE, 1.5 M LITHIUM SULFATE |
