5AN6
Crystal structure of Thermotoga maritima Csm2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 77.000, 77.000, 160.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.651 - 2.403 |
| R-factor | 0.2119 |
| Rwork | 0.210 |
| R-free | 0.24880 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.111 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.840 |
| Number of reflections | 21688 | |
| <I/σ(I)> | 12.63 | 3.64 |
| Completeness [%] | 98.3 | 94.9 |
| Redundancy | 6.24 | 5.03 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM SODIUM ACETATE PH 4.6 100MM CADMIUM CHLORIDE 21% PEG400 |
