5AN5
B. subtilis GpsB C-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-04 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 32.594, 36.203, 43.179 |
| Unit cell angles | 85.59, 89.32, 65.53 |
Refinement procedure
| Resolution | 29.659 - 1.200 |
| R-factor | 0.1458 |
| Rwork | 0.145 |
| R-free | 0.16340 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.058 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.040 | 1.220 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.050 | 0.130 |
| Number of reflections | 54744 | |
| <I/σ(I)> | 57.4 | 14.2 |
| Completeness [%] | 97.8 | 96.6 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M SODIUM FLUORIDE, 20% PEG 3350, pH 7.5 |
