5AN4
Crystal structure of the human 8-oxoguanine glycosylase (OGG1) processed with the CrystalDirect automated mounting and cryo-cooling technology
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 65 |
| Unit cell lengths | 106.071, 106.071, 47.287 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.520 - 1.600 |
| R-factor | 0.1851 |
| Rwork | 0.184 |
| R-free | 0.21427 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.952 |
| Refinement software | REFMAC (5.8.0123) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 27.000 |
| High resolution limit [Å] | 1.600 |
| Rmerge | 0.080 |
| Number of reflections | 40235 |
| Completeness [%] | 99.9 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM CITRATE, PH=5.5, 0.2 M AMMONIUM SULFATE, 24 % PEG 3350 |
