5AMV
Structural insights into the loss of catalytic competence in pectate lyase at low pH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.780, 88.120, 55.460 |
| Unit cell angles | 90.00, 110.41, 90.00 |
Refinement procedure
| Resolution | 51.980 - 1.570 |
| R-factor | 0.16063 |
| Rwork | 0.159 |
| R-free | 0.19283 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bn8 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.503 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.980 | 1.660 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 58741 | |
| <I/σ(I)> | 13.7 | 3.5 |
| Completeness [%] | 93.0 | 85.5 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 19% PEG 4K, 0.1M SODIUM ACETATE PH4, 0.2M AMMONIUM ACETATE |
