5AMU
IglE I39A,Y40A,V44A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 113 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.560, 53.780, 51.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.820 - 2.200 |
| R-factor | 0.2017 |
| Rwork | 0.198 |
| R-free | 0.26499 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5amt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.718 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.070 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 10932 | |
| <I/σ(I)> | 11.4 | 3.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.6 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
