5AMR
Structure of the La Crosse Bunyavirus polymerase in complex with the 3' viral RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2014-07-27 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 102.000, 140.700, 162.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.657 - 2.570 |
| R-factor | 0.2062 |
| Rwork | 0.204 |
| R-free | 0.25230 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.634 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.010 | 0.090 |
| Number of reflections | 71095 | |
| <I/σ(I)> | 11.2 | 1.28 |
| Completeness [%] | 94.7 | 97.4 |
| Redundancy | 3.55 | 3.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1M HEPES PH 7, 0.2M SODIUM CITRATE DIHYDRATE, 0.3M AMMONIUM SULPHATE, 15% PEG 3350. |
