5AL7
N-terminal fragment of Drosophila melanogaster Sas-6 (F143D), dimerised via the coiled-coil domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Collection date | 2011-09-10 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 47.130, 64.740, 123.730 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 41.429 - 2.920 |
| R-factor | 0.1847 |
| Rwork | 0.183 |
| R-free | 0.21470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ENSEMBLE OF PDB ENTRIES 2Y3V A B D 2Y3W A B 3Q0X A B |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.055 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.430 | 3.000 |
| High resolution limit [Å] | 2.920 | 2.920 |
| Rmerge | 0.130 | 0.670 |
| Number of reflections | 15767 | |
| <I/σ(I)> | 12.1 | 2.6 |
| Completeness [%] | 97.4 | 96.4 |
| Redundancy | 5.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1M BICINE/TRIZMA MIX (PH 8.5), 20% W/V PEG 550MME, 10% W/V PEG 20K, 30 MM NANO3, 30 MM NA2HPO4, 30 MM (NH4)2SO4 |
