5AL6
Central Coiled-Coil Domain (CCCD) of Drosophila melanogaster Ana2. A natural, parallel, tetrameric coiled-coil bundle.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-26 |
| Detector | PILATUS |
| Spacegroup name | I 4 |
| Unit cell lengths | 33.270, 33.270, 74.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.360 - 0.800 |
| R-factor | 0.10681 |
| Rwork | 0.106 |
| R-free | 0.11564 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uo4 CHAIN A RESIS 2 TO 31 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.230 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.360 | 0.820 |
| High resolution limit [Å] | 0.800 | 0.800 |
| Rmerge | 0.040 | 0.550 |
| Number of reflections | 41451 | |
| <I/σ(I)> | 21.5 | 2.2 |
| Completeness [%] | 97.8 | 79.2 |
| Redundancy | 5.8 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100 MM HEPES MIX (71% PH 7.2, 29% PH 8.2), 42% PEG 600 |
