5AJU
Crystal structure of ligand-free phosphoribohydroxylase lonely guy from Claviceps purpurea in complex with phosphoribose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 78.970, 78.970, 144.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.150 - 2.500 |
| R-factor | 0.2159 |
| Rwork | 0.214 |
| R-free | 0.24330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ajt |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.628 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.150 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.120 | 1.410 |
| Number of reflections | 8286 | |
| <I/σ(I)> | 9.1 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.9 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM AMMONIUM TARTRATE, 100MM POTASSIUM SULFATE, 23% PEG3350, 17% GLYCEROL, 3% ETHYLENE GLYCOL |
