5AJB
X-ray structure of the RSL lectin in complex with Lewis X tetrasaccahride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-18 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.185, 58.630, 106.713 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.030 - 1.800 |
| R-factor | 0.14743 |
| Rwork | 0.146 |
| R-free | 0.18021 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bt9 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.870 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.030 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.080 | 0.370 |
| Number of reflections | 25606 | |
| <I/σ(I)> | 16.1 | 4.1 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 5.3 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 27% PEG 6K, 0.1 M TRIS, PH 8.5 |
