5AHL
Apo-form of the DeltaCBS mutant of IMPDH from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 144.837, 144.837, 116.868 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.476 - 1.951 |
| R-factor | 0.1664 |
| Rwork | 0.164 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dqw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.032 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.200 | 1.980 |
| High resolution limit [Å] | 1.650 | 1.890 |
| Rmerge | 0.080 | 0.480 |
| Number of reflections | 45333 | |
| <I/σ(I)> | 21.9 | 5.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.7 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HEPES 0.1 M PH 7.5, NACL 4.3 M |
