5AFS
structure of Zn-bound periplasmic metal binding protein from candidatus liberibacter asiaticus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-10-02 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 94.370, 94.370, 94.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 42.240 - 2.220 |
| R-factor | 0.15447 |
| Rwork | 0.153 |
| R-free | 0.18142 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cl2 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.083 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.220 | 2.330 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.050 | 0.160 |
| Number of reflections | 24473 | |
| <I/σ(I)> | 37.2 | 15.6 |
| Completeness [%] | 98.9 | 92.5 |
| Redundancy | 11.5 | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 0.1M SODIUM ACETATE TRIHYDRATE PH 4.6, 2M AMMONIUM SULPHATE |
