5AFQ
Crystal structure of RPC62 - RPC32 beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-09-29 |
| Detector | ADSC CCD |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 218.270, 218.270, 182.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.750 - 7.000 |
| R-factor | 0.2708 |
| Rwork | 0.268 |
| R-free | 0.29840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2xub |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.000 | 7.380 |
| High resolution limit [Å] | 7.000 | 7.000 |
| Rmerge | 0.060 | 0.620 |
| Number of reflections | 3668 | |
| <I/σ(I)> | 8.1 | 1.2 |
| Completeness [%] | 99.2 | 97.9 |
| Redundancy | 4.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 100MM ADA PH 6.5, 100MM LI2SO4, 14% PEG 4000 |
